Recombinant Protein Expression and Purification

Guild BioSciences has the facilities and experience to grow, harvest, and purify your recombinant protein. We have experience with multiple hosts, including Escherichia coli, Saccharomyces cerevisiae (baker’s yeast), and mammalian 3T3 cells. Guild BioSciences extensively uses polyhistidine tags from E. coli lysates in our research, but we are have also used a FLAG-tag, a maltose-binding protein (MBP) tag, and a SUMO/polyhistidine tag. In our biosensors research, we have successfully developed recombinant proteins, expressed and harvested them in yeast, and provided purified proteins to other researchers.

Guild BioSciences’ pilot plant facilitates transition from lab-scale to pilot-scale fermentation, including investigation of enhancements to processing steps and product yield. Pilot fermentation production plants range in capacity from 1L to 100L. Ultrafiltration and chromatographic methods are available for purification. The pilot plant is available to support our own bioscience initiatives and allow others to leverage our expertise in pilot scale fermentation and purification process.