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Guild Associates
Guild Associates

Proteome Analysis: Differential Proteomic Analysis

Using Phoretix 2D Evolution software

Proteome Analysis
SDS PAGE
2-DE
RAMR™
Differential
Hi-Res
After protein separation, GBS performs differential proteomic analysis using Phoretix 2D Evolution software (Nonlinear Dynamics). Minimal analysis includes spot detection, calculation of estimated molecular weights, and isoelectric point of each spot, and a normalized quantitative value for each spot. Advanced analysis also includes spot matching between different gels and differential comparison of equivalent spot abundance values. For experiments with multiple replicates per experimental group, averaged gels are synthesized and used for differential analysis. When multiple experimental groups are present, each experimental group is compared to a reference gel synthesized from the control gel(s). Before contacting GBS, please visit the sample preparation page for tips on maximizing the results of analysis.
For this service,
the customer provides:
Upon completion of this service,
the customer receives:
2DE gels
  • Control/reference gel
  • Experimental gel
Operational Options
  • Replicate gel pair(s) for synthesizing average gels
  • Additional experimental samples
  • Gel size: mini (5x7 cm), Std (16x16 cm), large (26x20 cm)
Basic Report
  • Isoelectric point and molecular weights
  • Spot abundance values
  • Spot matching
  • Differential expression comparison using computer analysis and single gel pair
Pricing List
Base Price
$360 for each gel pair
For option pricing contact GBS by email, contact form, or by downloading our custom quote form.

*The customer is required to inform GBS if source material contains biological toxins, highly toxic reagents, or potentially infectious material, and if there are any special handling requirements. Analysis of radioactive material is not supported.
 
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