GBS will perform two-dimensional electrophoresis (2DE) on sample material provided by the customer.* The first dimension is conducted with immobilized pH gradient (IPG) strips using a Amersham Biosciences Multiphor II system. The system used for the second dimension depends on which size gel format is required. For minigel format, a Biorad Protean III system is used; for standard format a Hoeffer 600 system is used, and for large format an Ettan Daltsix (Amerisham Biosciences) system is used. Customer may submit sample material ready for analysis or raw materials including tissues, tissue cultures, cell lines, bacteria, yeast and other microorganisms, and GBS will process the raw material for an additional fee. (Organisms requiring special procedures for cellular disruption have an additional fee).

If the customer wishes to conduct initial sample disruption in-house, GBS can mail its optimized denaturing buffer cocktail for final sample dilution for a minimal charge. If samples are provided in denaturing buffer cocktail (ours or the customers) the total protein value for each sample will need to be provided to ensure proper loading quantities.

If GBS does the sample disruption total protein will be calculated for an additional charge. For isoelectric focusing (first dimension) customers may choose from a variety of IPG strips of broad to very narrow pH ranges, and from four different strip lengths. For the second dimension, the customer may choose from several total acrylamide concentrations (range 8-18%) for gels with homogenous acrylamide percentages, or request custom gradient gels for an additional charge.

Additionally, customers may request 2DE under non-denaturing or non-reducing conditions in either dimension or both. Customer may choose from three different size formats, and several different total protein, and specialized protein staining methods. Results are returned as a JPEG image, with a description of the running conditions.

You send us:
Cell lysate*
A list of sample buffer components
Total protein value for samples
Note: Extra charge if we have to lyse and separate tissue

We send you:
Base Report includes:
Gel images (1 gel, standard format - 11 cm strips, 16x16 cm gel)
Running conditions

Operational Options::
Mini gel format (5x7 cm)
Large gel format (26x20 cm)
pI - Isoelectric Point & Molecular weight values
Specialized staining
Non-reducing single dimension
Non-reducing both dimensions
Non-denaturing single dimension
Non-denaturing both dimensions
Dried gel returned
Protein identification w/mass spectrometry

Base Price is $170 for each sample.
Call or Email for option pricing.
1-888-704-4273
Contact us by clicking here.

*The customer is required to inform GBS if source material contains biological toxins, highly toxic reagents, or potentially infectious material and any special handling. 2DE analysis of radioactive material is not supported.