Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) is a procedure
used to separate protein mixtures on the basis of electrical charge and molecular
weight. At GBS, we understand that different proteins, starting materials, and end
uses require flexibility in the details of the SDS PAGE protocol. For that reason,
we offer various options to accommodate your specific needs, including a range of
acrylamide concentrations, multiple gel size formats, several different protein
stains, non-reducing SDS PAGE, and a non-denaturing version of SDS PAGE for proteins
that need to retain their functional abilities after analysis. Additional analyses
are also available.
Two-dimensional electrophoresis (2DE) is one of our specialties; the
Guild BioSciences Team offers more than
10 years of experience working with and sharpening this particular technique. During
2DE, your proteins are fractionated on the basis of isoelectric point (dimension
one) and then subjected to SDS PAGE (dimension two). You can submit prepared samples
or a variety of raw materials for 2DE analysis and we provide numerous options in
both dimensions to tailor the process to your particular interests.
Rapid Analysis Meso-Resolution (RAMR™) is a GBS innovation in protein profiling.
This pioneering process incorporates a proprietary pre-fractionation method, which
we use to amplify low-abundance protein signals, while hastening your project’s
processing time. The result – more data in less time. It is an excellent choice
for preliminary proteomic screening when proteins are abundant and have relatively
strong differential expression. Please note that RAMR™ protein profiling is currently
only available if your protein mixture is water-soluble.
Once a 2DE separation has been performed, more protein details can be gathered from
the gel. Our differential proteomic analysis service involves a series of computer-based
applications that reveal important information about your protein material. Using
Phoretix 2D Evolution software (Nonlinear Dynamics), we can generate a variety of
reports related to spot detection, characterization, and comparison as well as intra-
and inter-experimental group analyses.
Relative to standard 2DE outcomes, high-resolution proteomic analysis results in
improved sensitivity and superior resolution of your protein fractions. Our enhanced
results are due to the application of an optimized chromatographic pre-fractionation
prior to 2DE analysis. Additionally, we can work with you to develop customized
chromatographic pre-fractionations, if desired.