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Guild Associates
Guild Associates

Proteome Analysis: Hi-Res Proteomic Analysis Using Pre-Fractionation

Enhanced Proteomic Analysis

Proteome Analysis
SDS PAGE
2-DE
RAMR™
Differential
Hi-Res
For the high resolution proteomic analysis, GBS provides an optimized chromatographic pre-fractionation (also used in the RAMR protein profiling procedure), followed by 2DE of the isolated fractions. One of the advantages of this procedure is that it generates three 2DE gels per sample rather than one gel per sample. Because there is relatively little overlap between the different fractions, the enriching effects of the pre-fractionation allow greater sensitivity and resolution compared to standard 2DE and other pre-fractionation procedures. Also, if requested, customized chromatographic pre-fractionations can be developed through collaboration between the customer and GBS. High-resolution proteomic analysis is currently only available for water-soluble protein mixtures. Before contacting GBS, please visit the sample preparation page for tips on maximizing the results of analysis.
For this service,
the customer provides:
Upon completion of this service,
the customer receives:
Cell lysate* (tissue lysing and separation can be conducted at GBS for an extra charge)
Buffer composition
Total protein content
Operational Options
  • IEF/SDS PAGE conditions (non-reducing, non-denaturing, percent acrylamide, IPG strips)
  • Gel size: mini (5x7 cm), Std (16x16 cm), large (26x20 cm)
  • Staining: Coomassie, silver, SyproRuby, specialized
Basic Report
  • Gel images - 16 x 16 cm (3 gels - Std format)
  • Running conditions
Optional Reports
  • Isoelectric point (pI) and molecular weights
  • Dried gel returned
  • Protein identification with mass spectrometry
Pricing List
Base Price
$450 for each sample
For option pricing contact GBS by email, contact form, or by downloading our custom quote form.

*The customer is required to inform GBS if source material contains biological toxins, highly toxic reagents, or potentially infectious material, and if there are any special handling requirements. Analysis of radioactive material is not supported.
 
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