GBS will perform single or multiple chromatographic fractionations for the purification of customer selected proteins. GBS uses a Waters HPLC system for all standard chromatography separations. The customer must provide a means or method of identifying the protein of interest such as biochemical attributes (i.e. isoelectric point, molecular weight, biochemical assays -i.e. enzymatic assay- or appropriate antibody for western blots). If biochemical assays are used, a complete protocol and a list of necessary reagents must be provided by the customer. Biochemical assay and western blot identification incur additional charges determined on a case by case basis. If no biochemical information on the protein of interest is available, optimization runs may be necessary to effectively isolate the desired proteins.

GBS will also purify polyhistidine tagged recombinant proteins from E. coli lysates. GBS removes a small aliquot of the lysis for SDS PAGE analysis, then centrifuges lysate to separate membrane and aqueous fractions, and conducts SDS PAGE to determine to which fraction the recombinant protein partitions. Then Ni chromatography column is applied to the appropriate fraction to isolate the recombinant product. SDS PAGE is used to monitor the chromatography. If recombinant product still contains unacceptable protein contaminants, additional chromatography steps can be applied to improve purity. Purified protein can be concentrated, and desalted, total protein determined and percent purity estimated at additional cost.

GBS will also purify recombinant proteins having other tags ( GST fusion etc), however, since other recombinant chromatography systems are not maintained inhouse the cost will be higher.

Note: recombinant proteins vary considerably in their susceptibility to such purification schemes. GBS does not guarantee purification success, but will work with the client to achieve the highest efficiency possible within its means.

You send us:
Buffer composition*
Sample complexity
Total protein content
Means of identifying proteins of interest
Recombinant or Non-Recombinant

We send you:
For natural proteins (non-recombinant):
  Chromatogram from LC run
  SDS PAGE Gel image of peak
  Purified protein (freeze-dried unless otherwise specified)
For recombinant proteins:
  SDS PAGE gels that follows the purification steps.
  Purified protein (freeze-dried unless otherwise specified)

Custom Pricing**
Call or e-mail for Quote.
1-888-704-4273
Contact us by clicking here.

*Customer is required to inform GBS if source material contains biological toxins, or potentially infectious material and any special handling requirements. PAGE analysis of radioactive material is not supported.
**Final price quote will include procurement of necessary reagents for biochemical assays. (Reagent delivery time may affect analysis report time.)