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Protein Analysis: Characterization

Protein Characterization

Protein Analysis
Isolation and Purification
Characterization
The GBS characterization service involves determining the physiochemical parameters of the protein of interest through various biochemical analyses. Fully and partially purified proteins can be characterized, but for partially purified materials, the customer is required to provide a means of identifying the protein(s) of interest.
Characterization results are returned in a report format, which includes estimates of molecular weight, isoelectric point, and relative hydrophobicity. (See the 2DE page for a system description.) Relative hydrophobicity is determined using an analytical Hydrophobic Interaction Column, calibrated with standard marker proteins.
For customers interested in a more detailed characterization, supplemental information on post-translational modification (glycosylation, phosphorylation, presence of cysteine residues, presence of disulfide bonds) and protein identification by tryptic digestion and mass spectrometry are available for additional charges. Before contacting GBS, please visit the sample preparation page for tips on maximizing purification results.
For this service,
the customer provides:
Upon completion of this service,
the customer receives:
Fully or partially purified protein*
Method of identification for Protein (if submitting partially purified material)
Choice of procedural options:
  • 7 cm strip (10 x 8 cm gel)
  • 18 cm strip (26 x 20 cm gel)
  • 24 cm strip (26 x 20 cm gel)
Basic Report
  • Molecular weight
  • Isoelectric point
  • Relative hydrophobicity
Optional Reports
  • Glycosylation
  • Phosphorylation
  • Identification (via Mass Spec)
  • Custom characterization
Pricing List
Base Price
$400 for 3 gels OR $150/gel
Contact GBS by email, contact form, or by downloading our custom quote form.

*The customer is required to inform GBS if source material contains biological toxins, highly toxic reagents, or potentially infectious material, and if there are any special handling requirements. Analysis of radioactive material is not supported.
 
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